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第130期 東曹色譜產(chǎn)品在病毒關(guān)聯(lián)藥物和疫苗研發(fā)中的應(yīng)用

2020年07月01日來源:管理員
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第130期 東曹色譜產(chǎn)品在病毒關(guān)聯(lián)藥物和疫苗研發(fā)中的應(yīng)用

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病毒是一種個體微小,結(jié)構(gòu)簡單,只含一種核酸(DNA或RNA),必須在活細胞內(nèi)寄生并以復(fù)制方式增殖的非細胞型生物。

病毒是一種非細胞生命形態(tài),它由一個核酸長鏈和蛋白質(zhì)外殼構(gòu)成,病毒沒有自己的代謝機構(gòu),沒有酶系統(tǒng)。因此病毒離開了宿主細胞,就成了沒有任何生命活動、也不能獨立自我繁殖的化學(xué)物質(zhì)。它的復(fù)制、轉(zhuǎn)錄和轉(zhuǎn)譯的能力都是在宿主細胞中進行,當(dāng)它進入宿主細胞后,它就可以利用細胞中的物質(zhì)和能量完成生命活動,按照它自己的核酸所包含的遺傳信息產(chǎn)生和它一樣的新一代病毒。

一、病毒和疫苗的特性

※疫苗

·蛋白質(zhì)作為表面抗原

·天然蛋白、多糖或重組蛋白

·類似于蛋白質(zhì)分離的純化策略

·純化用到的分離模式SEC、IEC、HIC和AFC

※病毒

·巨大的生物分子復(fù)合粒子

·流感病毒:100-120nm;腺病毒:80nm;天花病毒:30nm

·不同純化策略對蛋白質(zhì)的分離有不同的影響

二、疫苗用色譜填料

※尺寸排除色譜法(SEC)

·病毒分離需要大孔徑的填料

病毒尺寸:100nm----TOYOPEARL HW-65,Sepharose 6 FF

病毒尺寸:200nm---- TOYOPEARL HW-75,Sepharose 4 FF

·填料的剛性對裝柱更為重要

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TOYOPEARL比Sepharose基質(zhì)具有更好的剛性和穩(wěn)定性。

※離子交換色譜法(IEC)

·大孔徑的填料:TOYOPEARL 650系列

·? 接枝型的聚甲基丙烯酸甲酯,分離效果會更好,如GigaCap Q-650M,GigaCap S-650M,SuperQ-650M

※疏水層析(HIC)

·不同填料的疏水性有差異

·疏水性較低的填料可能更有效分離病毒

·DNA在HIC填料上的保留較差

※親和層析(AFC)

·染料:TOYOPEARL AF-Blue-650M

·肝素:TOYOPEARL AF-Heparin-HC-650M

·其他蛋白質(zhì)固定化AFC樹脂

?? 在這些關(guān)聯(lián)藥物的研發(fā)、評價、純化精制中,TSKgel液相色譜柱和Toyopearl層析填料產(chǎn)品有著相當(dāng)多的應(yīng)用實例


TSKgel色譜柱分析柱的應(yīng)用實例

Year

Mode

TSKgel Column

Target,keyword

Title

Citation at web site

2020

SEC

TSKgel G5000PWXL

Coronavirus protein/

冠狀病毒蛋白

Characterization of the SARS-CoV-2 Spike in an Early Prefusion ? Conformation/早期融合前構(gòu)象中SARS-CoV-2峰的表征

https://www.biorxiv.org/content/10.1101/2020.03.16.994152v1.full

2020

IEC

TSKgel DNA-STAT

PS-oligonucleotide /

PS-寡聚核苷酸

Condition Study of Phosphorothioate Oli gonucleotides Separation by High- performance Anion Exchange Chromatography ? /硫代磷酸寡聚核苷的陰離子高效液相色譜分析的條件研究

Poster presentation in Asia? TIDES 2020

2019

SEC

TSKgel UP-SW2000

Oligonucleotide /

寡聚核苷酸

Analysis of olignucleotide by SEC /寡聚核苷酸的分子尺寸排阻色譜分析

https://www.separations.us.tosohbioscience.com/HPLC_Columns/id-8694/TSKgel_UP-SW2000?inval=1&Target=uJzmmWx7eqe3bbXcid9Eq/UAKSx5S2pFX8hW+KqJ6YMeIGVvJ+3p89zBvATw8mUQ7DWvS7VERS6O+kyfR/mkqQ==

2019

RPC

IEC

TSKgel ODS-120H

DNA-STAT

Oligonucleotide /

寡聚核苷酸

Examination of analytical conditions for separation of oligonucleotides ? for LC/MS /液相色譜進行寡聚核苷酸分析條件的檢驗

Poster presentation in HPLC Kyoto, 2019

2017

SEC

TSKgel G6000PWXL

G5000PWXL

Conjugated vaccine /

結(jié)合疫苗

Determination of free polysaccharide in Vi glycoconjugate vaccineagainst typhoid fever /傷寒疫苗中游離多糖的測定

https://www.sciencedirect.com/science/article/pii/S0731708516312511?via%3Dihub

2017

SEC

TSKgel G5000PWXL

Virus-like particle /

類病毒顆粒(VLP

Quantification and characterization of virus-like particles by ? size-exclusion chromatography and nanoparticle tracking analysis / 通過尺寸排除色譜和納米顆粒跟蹤定性和定量分析類病毒顆粒

https://www.sciencedirect.com/science/article/pii/S0021967316317514

2016

SEC

TSKgel G3000PWXL

Vaccine/疫苗

Immunogenicity and protective efficacy of rotavirus VP8 fused to ? cholera toxin B subunit in a mouse model/輪狀病毒VP8與霍亂毒素B亞單位融合在小鼠模型中的免疫原性和保護作用

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137547/

2016

SEC

TSKgel G6000PWXL

Flu virus(VP)/流感病毒

Size distribution analysis of influenza virus particles using size ? exclusion chromatography /流感病毒顆粒大小分布的分子尺寸排阻色譜分析

https://www.sciencedirect.com/science/article/pii/S0021967316311335

2015

SEC

TSKgel G5000PWXL

G4000PWXL

Conjugated vaccine/結(jié)合疫苗

Development and characterization of Haemophilus influenzae type b conjugate ? vaccine prepared using different polysaccharide chain lengths /不同多糖鏈長流感嗜血桿菌B型偶聯(lián)疫苗的研制與表征

https://www.sciencedirect.com/science/article/pii/S0264410X15004958?via%3Dihub

2014

SEC

TSKgel G5000PWXL-CP

Bacteriophage particle/噬菌體顆粒

Size exclusion-based purification and PCR-based quantitation of MS2 ? bacteriophage particles for environmental applications/用于環(huán)境應(yīng)用的MS2噬菌體顆粒的儲存排阻純化與PCR定量

https://www.sciencedirect.com/science/article/pii/S0166093414004704?via%3Dihub

2013

SEC

TSKgel G5000PWXL-CP

Virus particle/病毒顆粒

A Gel Filtration -Based Method for the Purification of Infectious ? Rotavirus Particles for Environmental Research Applications /一種用于環(huán)境研究應(yīng)用的感染性輪狀病毒的凝膠過濾純化方法

https://link.springer.com/article/10.1007/s12560-013-9122-4

2013

HIC

TSKgel Butyl-NPR

DNA vaccine/DNA 疫苗

Antibiotic-free production of a herpes simplex virus 2 DNA vaccine in a high yield ? cGMP process /無抗生素生產(chǎn)單純皰疹病毒2DNA疫苗的高產(chǎn)cGMP過程

https://www.tandfonline.com/doi/full/10.4161/hv.25048

2008

SEC

TSKgel G4000PWXL

Flu vaccine/流感疫苗

Development of physicochemical methods for analysis of pandemic ? influenza vaccine/大流行性流感疫苗理化分析方法的發(fā)展

https://journal.lib.uoguelph.ca/index.php/surg/article/view/420

2007

IEC

TSKgel DNA-NPR

Plasmid DNA/質(zhì)粒DNA

Separation of topological forms plasmid DNA by anion -exchange HPLC: Shifts ? in elution order of linear DNA/陰離子交換高效液相色譜法分離質(zhì)粒DNA的拓撲結(jié)構(gòu)

https://www.sciencedirect.com/science/article/pii/S1570023207002747

2007

SEC

TSKgel G5000PWXL

Conjugated vaccine /結(jié)合疫苗

Quality and Immunogenicity of MenAfriVac, a Conjugate Vaccine Against ? Meningococcal Group ADisease/腦膜炎球菌 a群疾病的結(jié)合疫苗MenAfriVac的質(zhì)量和免疫原性

https://www.meningvax.org/files/PharmaPhase2PosterSept07.pdf

2007

SEC

TSKgel G5000PWXL

Flu vaccine /流感疫苗

A Retrospective Study on the Quality of Haemophilus influenzae type b ? Vaccines Used in the UK Between 1996 and 2004 / 1996年至 2004 年英國使用的 B型流感嗜血桿菌疫苗的質(zhì)量進行回顧性研究

https://www.tandfonline.com/doi/abs/10.4161/hv.3.5.4352

2005

SEC

TSKgel G-DNA-PW

G6000PWXL

Plasmid DNA/質(zhì)粒DNA

High-speed chromatograph purification of plasmid DNA with a customized biporous ? hydrophobic adsorbent/用定制的雙孔疏水吸附劑對質(zhì)粒DNA進行高速色譜純化

https://www.sciencedirect.com/science/article/abs/pii/S1369703X05002044

2003

SEC

TSKgel G5000PWXL

G4000PWXL

Liposome/脂質(zhì)體

Characterization of loaded liposomes by size exclusion chromatography/已包載脂質(zhì)體的分 子尺寸排阻色譜分析

https://www.sciencedirect.com/science/article/pii/S0165022X03000599?via%3Dihub

?

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Toyopearl層析填料的分離純化應(yīng)用實例

Year

Mode

TSKgel Column

Target,keyword

Title

Citation at web site

2019

HIC

PPG-600M

Flu virus/流感病毒

Hydrophobic-interaction chromatography for purification of influenza A ? and B virus / 疏水層析純化甲型和乙型流感病毒

https://www.sciencedirect.com/science/article/pii/S1570023219301655?via%3Dihub

2019

IEC

TSKgel SuperQ-5PW

Nucleotides/核苷酸

Generic anion -exchange chromatography method for analytical and ? preparative separation of nucleotides in the development andmanufacture of ? drug substances /通用陰離子交換色譜法用于核酸藥物的研發(fā)、生產(chǎn)中的分析

https://www.sciencedirect.com/science/article/pii/S0021967318315255?via%3Dihub

2018

IEC

TSKgel SuperQ-5PW

siRNA

Comprehensive evaluation of canonical versus Dicericericer-substrate ? siRNA in vitro and in vivo

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3285942/

2018

SEC

HW-65F

Virus-like particle (VLP) / 類病毒顆粒

Dengue virus-like particles mimic the antigenic properties of the ? infectious dengue virus envelope /等革類病毒顆粒模擬感染性登革病毒包膜的抗原特性

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5879749/

2017

HIC

Butyl-650M

BBC vaccine/BBC疫苗

Multi-subunit BCG booster vaccine GamTBvac: Assessment of ? immunogenicity and protective efficacy in murine and guinea pig TB models/多亞單位卡介苗增強疫苗GamTBvac:小鼠和豚鼠結(jié)核病模型免疫原性和保護效果的評估

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5409163/

2016

HIC

Butyl-650M

Malaria vaccine/瘧疾疫苗

Production, Quality Control, Stability and Pharmacotoxicity of a ? Malaria Vaccine Comprising Three Highly Similar PfAMA1 Protein Molecules to Overcome ? Antigenic Variation/由三種高度相似的PfAMA1蛋白分子組成(克服抗原變異)的瘧疾疫苗的生產(chǎn)、質(zhì)量控制、穩(wěn)定性和藥物毒性

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0164053

2016

IEC

NH2-750F

GigaCap Q-650M

Flu virus/流感病毒

Mono- and polyprotic buffer systems in anion- exchange chromatography ? of influenza virus particles /流感病毒顆粒陰離子交換層析中單、多質(zhì)子緩沖體系的研究

https://www.sciencedirect.com/science/article/pii/S0021967316304708?via%3Dihub

2015

AFC

AF-Heparin 650M

Pertussis vaccine /百日咳疫苗

Single Amino Acid Polymorphism s of Pertussis Toxin Subunit S2 (PtxB) ? Affect Protein Function /百日咳毒素亞基S2(PtxB)單氨基酸多態(tài)性影響蛋白功能

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4573519/

2013

IEC

DEAE-650M

HBV-VLP/HBV 類病毒顆粒

Purification of hepatitis B surface antigen virus-like particles from ? recombinant Pichia pastoris and in vivo analysis of their immunogenic ? properties /從重組畢赤酵母中純化乙型肝炎表面抗原樣病毒顆粒,并在體內(nèi)分析其免疫原性

https://www.sciencedirect.com/science/article/pii/S1570023213005126?via%3Dihub

2012

HIC

Phenyl-600M

HBV vaccine/乙肝疫苗

Novel membrane extraction procedure for the purification of hepatitis B ? surface antigen from Pichia pastoris/新型膜提取法從畢赤酵母中分離純化乙型肝炎表面抗原

https://www.sciencedirect.com/science/article/pii/S1570023212002115?via%3Dihub

2012

IEC

DEAE-650M

Anti-viral component/抗病毒成分

Isolation of antiviral components against Influenza from food in ? Yamagata (report Japanese) /從山形縣食物中分離抗流感病毒成分

http://www.eiken.yamagata.yamagata.jp/pdfshohou/shohou45.pdf

2009

HIC

(HAC)

Phenyl-650M

(Ca++Pure-HA)

Botlinum vaccine/肉毒疫苗

Bivalent recombinant vaccine for botlinum neurotoxin types A and B ? based on a polypeptide comprising their effector and translocation domains ? that is protective against the predominant A and B subtypes /基于包含其效應(yīng)域和易位域的多肽肉毒桿菌神經(jīng)毒素A型和B型的雙價重組疫苗

https://www.ncbi.nlm.nih.gov/pubmed/19398544

2006

IEC

DEAE-650M

HBV vaccine/乙肝疫苗

Expression in and purification of Hepatitis B surface antigen (S-protein) ? from methylotrophic yeast Pichia pastoris/乙型肝炎表面抗原(S-蛋白)的表達及純化

https://www.sciencedirect.com/science/article/pii/S107599640600045X?via%3Dihub

2004

IEC

DEAE-650M

Adenovirus/腺病毒

Development and optimization of an adenovirus production process/腺病毒生產(chǎn)工藝的開發(fā)和優(yōu)化

https://onlinelibrary.wiley.com/doi/10.1002/jgm.503

2002

IEC

SEC

SP-650M,HW-65S

Botlinum vaccine/肉毒疫苗

Mucosal immunisation with Clostridium botulinum type C 16 S toxoid and ? its non-toxic component / C16 S-型肉毒桿菌及其無毒成分的粘膜免疫

https://www.microbiologyresearch.org/content/journal/jmm/10.1099/0022-1317-51-10-813

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???? 北京慧德易作為東曹(TOSOH)公司產(chǎn)品的一級代理,竭誠為您服務(wù)!可提供東曹公司聚合物基質(zhì)層析填料的小包裝試用,歡迎索取!如需試用或者更詳細的資料,請聯(lián)系我們。


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